General description

Full-length, recombinant, human, PKCε expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCε and its regulation. PKCε is a Ca²⁺-independent and phospholipid-dependent enzyme.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Newton, A.C. 1995. J. Biol. Chem.270, 28495.Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.Nishizuka, Y. 1992. Science 258, 607.Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.

Packaging

20 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC_ε Peptide Substrate (catNO=539562">Cat. No. 539562) per min at 30°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)